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Journal: Structure (London, England : 1993)

Article Title: Pih1p-Tah1p Puts a Lid on Hexameric AAA+ ATPases Rvb1/2p

doi: 10.1016/j.str.2017.08.002

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Rosetta (DE3) EMD Millipore (Novagene) Cat# 70954 Chemicals, Peptides, and Recombinant Proteins Q5 site-specific mutagenesis kit New England Biolab Cat# E0554S BS 3 -d 0 /d 4 Thermo Scientific Cat# 21590/21595 Glutaraldehyde solution Sigma Aldrich Cat# G7651–10ML ProteoExtract all-in-one trypsin digestion kit Calbiochem Cat# 650212 Uranyl Formate Electron Microscopy Sciences Cat# 22450 Deposited Data Experimental map This paper EMD-8839 Recombinant DNA pHRvb1 This paper pRvb2 This paper AII-Rvb2p This paper pHPih1-Tah1 This paper pHNop58_447 This paper Software and Algorithms UltraScanIII ( Demeler, 2010 ) http://www.ultrascan3.uthscsa.edu/ US-SOMO ( Brookes et al., 2010 ) http://www.somo.uthscsa.edu/ Zeno ( Mansfield et al., 2001 ) https://doi.org/10.18434/T48W2J Appion ( Lander et al., 2009 ) http://www.appion.org ACE ( Mallick et al., 2005 ) http://nramm.scripps.edu/software/ace http://graphics.ucsd.edu/spmallick/research/ace CTFFIND ( Rohou and Grigorieff, 2015 ) http://grigoriefflab.janelia.org/ctf FindEM ( Roseman, 2004 ) Relion ( Scheres, 2012 ) http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page Frealign ( Grigorieff, 2016 ) http://grigoriefflab.janelia.org/frealign ProteoWizard MSConvert ( Chambers et al., 2012 ) http://proteowizard.sourceforge.net Chimera ( Pettersen et al., 2004 ) http://www.cgl.ucsf.edu/chimera/ Other Ni-NTA agrose Qiagen Cat# 30230 Superdex 200 PC 3.2/30 GE healthcare Cat# 17–1089-01 Hiload 16/60 superdex 200 GE healthcare Cat# 17–1069-01 Superdex 200 Increase 10/300 GL GE healthcare Cat# 28–9909-44 Mono Q 5/50 GL GE healthcare Cat# 17–5166-01 R 2/2 200 mesh Cu holey carbon grids Quantifoil Carbon-only 400 mesh grids Cu Electron Microscopy Sciences Cat# CF400-cu Open in a separate window KEY RESOURCE TABLE

Techniques: Recombinant, Mutagenesis, Electron Microscopy, Software